1021
P47
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Ùǰ S rDNA-PCR ĒúąǰDGGE
The Detection of Bacteria from Bovine Mastitis Raw Milk using 16S rDNA-PCR and DGGE
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Denaturing Gradient Gel Electrophoresis óïüŠ
ćÙüćöĀúćÖĀúć÷×ĂÜĒïÙìĊ
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÷ĕéš
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ǰ #BDJMMVT
sp.
4UBQIZMPDPDDVTǰ
sp.
4QIJOHPNPOBT
sp.ǰĒúą uncultured bacteria đߊ
îǰuncultured
-BDUPCBDJMMVT
sp.
uncultured
.JDSPDPDDVT
sp. uncultured bacterium uncultured
4QIJOHPNPOBT
spǰĒúą Bacterium
enrichment
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îǰ16S rRNA đìÙîĉ
ÙǰDGGE
Abstract
The objectives of this study is to detect bacterial diversity from high somatic cell and low
somatic cell raw milk samples. The V3 region of 16S rRNA gene was amplified by nested PCR and
were separated by Denaturing Gradient Gel Electrophoresis method. The result showed that
#BDJMMVT
sp.
4UBQIZMPDPDDVTǰ
sp.
4QIJOHPNPOBT
sp and many uncultured bacteria such as uncultured
-BDUPCBDJMMVT
sp. uncultured
.JDSPDPDDVT
sp. uncultured bacterium uncultured
4QIJOHPNPOBT
sp.
and Bacterium enrichment culture
Keywords:
Bacterial Population, Raw Milk, Mastitis, 16S rRNA Gene and DGGE
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Corresponding author: Email address:
