3
J
K$
E? ก p
(tetracycline) Agarose gel 3% hydrogen peroxide Lysozyme 4-methyllumbelliferyl-
β
-D-N,N}-diacetylchitobiose (4-MUG) 10 mM Tris-HCl pH 8.0 10 mM MgCl
2
0.5 M sucrose
10% glycerol
ก B #
ก' &H
ก
@
; ; < ! ? 25 ก p กG; < @B
!9B
; < MRS M 225
. Q >< Q ก C =:
G =@:
; <Pq
37 ;
@
MR
24 !:
A C ก B
=F
C9
;C GG 10 = < F
EM
A? I@
ก @:
C G p < ; < @B
!9B
; Qy
TS
MRS F
EMG =@:
; <Pq
37 ;
@
MR
48 !:
A > G ก 5-10 % CO
2
9
;กA A @
=@:
G ;G K E @
!9B
;;9:
C eQDB
C ก B
F
EM=F
>< MR
!9B
;G =IJ
9:
;C F
!9B
;EM=? ;GH=IJ
C = @
(antimicrobial spectrum) ? I@
Q@
? !9B
; (cross streak)
ก B antimicrobial spectrum B B $#I
F
A A @
Q; G =@
@
ก=@:
? 9
;กE? ( !9B
;=? ;G) EMQ@
? MR
ก C ; < @B
!9B
; Qy
TS
F
EMG =@:
; <Pq
37 ;
@
MR
48 !:
A C ก B
F
A A @
Q; C = @
;9:
K =@:
>! MR
?! @
EMQ@
?
MR
B
กกG !9B
;=? ;G
G =@:
; <Pq
37 ;
@
MR
24 !:
A
ก ก C eQ; C = @
=@:
>!
MR
?! @
(
;@
?> < Q;C = @
G =IJ
)
ก
! &B $ ' &
(UV)
@B
L. plantarum
TISTR050 > ; < @B
!9B
; < MRS M 100
G > C
!9B
;
F
EM E > < กF
? ; E A; (UV) MR
10 =@
C ก B
F
EM=F
C9
;C GG 10 = F
=@:
C9
;
C ?G 10
-6
10
-7
EMก C G ; < @B
!9B
; Qy
MRS G =@:
; <Pq
37 ;
@
MR
48 !:
A
> G ก 5-10 % CO
2
?=GA A @
? ; < @B
!9B
;กD:
Qy
< SD M 5
=@:
@
@
?! @
(
C. albicans
TISTR 5239) p ; q
G =@:
; <Pq
37 ;
@
MR
24 !:
A
ก > (clear zone) Q;
ก G B
@
?! @
ก
! B ก PC
' QC
$#I
& H
@B
L. plantarum
TISTR 050 > ; < @B
!9B
; < MRS M 100
G =@:
; <Pq
37 ; -
@
> q
G !9B
; GG Q (shaking incubator) =@:
y
;G 150 ;G ; =@
MR
M 6 !:
A
ก กy
G ก; !9B
;? ก Mm
< @:
=@:
y
;G 5000 ;G ; =@
MR
10 =@
=@:
; <Pq
4 ;
@
F
ก; !9B
;EM>! MR
C G (host)
@B
E. coli
DH
α
=@:
@
@
E
pHYB43 > ; < @B
!9B
; < LB M 5
G =@:
; <Pq
37 ;
@
> q
G !9B
; GG Q =@:
y
;G 150 ;G ; =@
MR
24 !:
A F
EM ก??@
;y
;?
!? ก??@
;y
; QIAgen (QIAgen, Germany)
F
F
Q; G = C ก B
F
EMp กG C G 9:
;
F
Q q
? I@
electroporation (Alegre
et.al.
, 2004.) ? 9
;ก C G
L. plantarum
TISTR 050 =@:
@
@
E
(transformants) G C ; < @B
!9B
; Qy
p tetracycline
ก B ก B ก ' B
I
H
F
A A @
Q; transformants =@:
? 9
;กE?EMQ @B
? = ก < ?=G?
4-MUG
F
EM?q
C?
9
; @
w
(light blue fluorescence) P > 9:
; UV transilluminator C ก B
F
EM ก??@
;y
;? !? ก?
?@
;y
;
F
EM 9
ก @
?@
;y
;> ; ก A (
, 2531; Maniatis
et.al.
, 1982)
