5
? 9
;ก transformants E?CF
2 E;A =@:
L ? ;;กQ; @
E
9:
;=? ;G? I@
Q @B
ก <y
LGQ; ?@
;y
;E?C ก transformants 9:
; F
EM :
> ; ก A ? P =@:
1
A B C D
lane A - ?@
;y
;C ก
L. plantarum
lane B - ?@
;y
;C ก transformant E;A 1
lane C - ?@
;y
;C ก transformant E;A 2
lane D - @
E
pHYB43
S ! 1
q
M GG LG?@
;y
;=@:
E?C กก ก?C ก transformants (lane B
C). F
< q
ก !@B
( ) 9
; @
E
BF
@B
!9B
;C ก transformants
? H=IJ
C = @
;9:
K =@:
>! MR
?! @
E?E ก C ก
L. plantarum
TISTR
050 C G
ก <y
? H=IJ
> ก
M. luteus
TISTR 884 q
ก
L. plantarum
TISTR 050 C G
(
=@:
1) 9:
;=? ;G? I@
well diffusion (P =@:
2)
1
H=IJ
C = @
?! @
Q; BF
@B
!9B
; transformants 9:
;=? ;G? I@
well diffusion
clear zone (cm)
> C ก
S. aureus
B. subtilis
P. aeruginosa
M. luteus
C. albicans
E. coli
L. plantarum
1.5
1.5
2.0
2.2
-
1.2
transformant 1
1.4
1.4
1.5
2.5
-
1.2
transformant 2
1.4
1.7
1.8
2.4
-
1.2
BF
ก :
-
-
-
-
-
-
; < @B
!9B
; < MRS
-
-
-
-
-
-
L. plantarum
,
Lactobacillus plantarum
ATCC 8014 (TISTR 050);
S. aureus
,
Staphylococcus aureus
ATCC 25923
(TISTR 517);
B. subtilis
,
Bacillus subtilis
ATCC 6633 (TISTR 008);
P. aeruginosa
,
Pseudomonas aeruginosa
ATCC 9027 (TISTR 781);
M. luteus
,
Micrococcus luteus
TISTR 884 ;
C. albicans
,
Candida albicans
DSM 70014
(TISTR 5239);
E. coli
,
Escherichia coli
ATCC 25922 (TISTR 887); transformant 1
transformant 2,
L. plantarum
TISTR 050 =@:
@
@
E
E;A =@:
1
E;A =@:
2
