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Isolation of Xylanase-Producing Thermophilic Fungi
from Kuankang Hot Spring, Trang Province
ßĆ
÷ßîąǰÙÜèøÜÙŤ
1
úćüĆ
ú÷Ť
ǰßêćîîìŤ
2
ǰóùìåĉ
õøǰýč
õóú
3
ǰĒúąýč
õßĆ
÷ǰîĉ
êĉ
óĆ
îíŤ
3*
Chaichana Kongnarong
1
, Lawan Chatanon
2
, Preuttiporn Supaphon
3
and Supachai Nitipan
3*
ïìÙĆ
é÷Š
Ă
øćßĂïøš
Ăîǰ Thermophilic fungi ǰöĊ
øć÷ÜćîüŠ
ćÿćöćøëñúĉ
êđĂîĕàöŤ
ǰXylanase ìĊę
ìîĂč
èĀõĎ
öĉ
ÿĎ
Üĕéš
ǰ
thermostable xylanase ǰÿćöćøëđÝøĉ
âĕéš
Ĕîÿĉę
ÜĒüéúš
ĂöìĊę
öĊ
Ăč
èĀõĎ
öĉ
ÿĎ
ÜöćÖÖüŠ
ćǰ45 °C ǰÝč
éðøąÿÜÙŤ
×ĂÜÖćøýċ
ÖþćĔî
ÙøĆĚ
ÜîĊĚ
ÙČ
ĂǰÙĆ
éĒ÷ÖøćßĂïøš
ĂîìĊę
ÿćöćøëñúĉ
êđĂîĕàöŤ
XylanaseǰÝćÖïŠ
ĂîĚĞ
ćøš
ĂîÙüîĒÙÜǰÝĆ
ÜĀüĆ
éêøĆ
Üǰàċę
ÜÙĆ
éĒ÷ÖøćÝćÖ
êĆ
üĂ÷Š
ćÜǰĕöš
ǰĔïĕöš
ǰĒúąéĉ
îǰìĊę
öĊ
øŠ
ĂÜøĂ÷ÖćøëĎ
Ö÷Š
Ă÷ÿúć÷øĂïėǰïŠ
ĂîĚĞ
ćøš
Ăî đúĊĚ
÷ÜøćĔĀš
đÝøĉ
âïîÝćîĂćĀćøǰPotato
Dextrose Agar (PDA) ìĊę
Ăč
èĀõĎ
öĉ
ǰ°C đðŨ
îđüúćǰ5-7 üĆ
îǰîĞ
ćøćìĊę
ÙĆ
éĒ÷Öĕéš
ìéÿĂïÙüćöÿćöćøëĔîÖćøñúĉ
êđĂîĕàöŤ
XylanaseǰïîÝćîĂćĀćøǰCellulolysis Basal Media (CBM) ēé÷Ĕßš
Xylan đðŨ
îÿĆ
ïÿđêøìǰÿćöćøëÙĆ
éĒ÷ÖøćßĂïøš
Ăîĕéš
ìĆĚ
ÜĀöéǰ32 ĕĂēàđúêǰìĊę
đÝøĉ
âĕéš
ìĊę
Ăč
èĀõĎ
öĉ
ǰ ǰ°CǰöĊ
ÝĞ
ćîüîǰ7 ĕĂēàđúêìĊę
öĊ
ÙŠ
ćǰHydrolysis Capacity (HC value)ǰöćÖÖüŠ
ćǰ
1.2 ĒúąöĊ
Öĉ
ÝÖøøö×ĂÜđĂîĕàöŤ
öćÖìĊę
ÿč
éĔîüĆ
îìĊę
ǰ9 ĕéš
ĒÖŠ
ǰĕĂēàđúêǰKKH0418 öĊ
Öĉ
ÝÖøøö×ĂÜđĂîĕàöŤ
đìŠ
ćÖĆ
ïǰ42.7ǰU/mlǰ
ÙĞ
ćÿĞ
ćÙĆ
â
:ǰøćßĂïøš
ĂîǰïŠ
ĂîĚĞ
ćøš
ĂîǰÙüîĒÙÜ đĂîĕàöŤ
ĕàúćđîÿìîøš
Ăî
Abstract
Thermophilic fungi have been reported their ability to produce thermostable xylanase. They
can growth in high temperature environment above 45 °C. This research is aimed to isolate xylanase -
producing thermophilic fungi from Kuankang Hot Spring, Trang Province. The decay-wood,-leaf and soil
around the hot spring were sampled and isolated for fungi on Potato Dextrose Agar (PDA) at 45 °C for
5-7 days. The isolated fungi were tested for xylanase production on Cellulolysis Basal Media (CBM)
containing xylan as substrate. The result showed 32 isolates of thermophilic fungi grow on PDA. There
are 7 isolates having Hydrolysis Capacity (HC value) higher than 1.2 and KKH0418 showed highest
activity of xylanase of 42.7ǰU/ml after 9 day cultivation.
Keywords:
Thermophilic Fungi, Hot Spring, Kuankang, Thermostable Xylanase
1
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Üǰ
*
Corresponding author: Tel.: 084-0840207. E-mail address:
